Alpha-Amilase (alfa-amilase)ELISA Kit Salivar

1-1902
Salimetrics
96 ou 480 testes
Análises Salivares

Os kits da Salimetrics são desenvolvidos e especificamente designados para a pesquisa com SALIVA, e trazem resultados precisos e acurados para os biomarcadores nela presentes.

Kit ELISA designado para a determinação quantitativa de alpha-amilase em amostras de saliva.

  • Volume de amostra: 10 µL, 8 µL of X200 dilution
  • Sensibilidade: 0.4 U/mL
  • Tempo de ensaio: 30 minutos
  • Faixa de detecção: 5.1 - 500 pg/mL
  • Testes por unicata: 94
  • Testes por duplicata: 46

Referências

Granger, D.A., Kivlighan, K.T., El-Sheikh, M., Gordis, E., & Stroud, L.R. (2007). Salivary alpha-amylase in biobehavioral research: Recent developments and applications. Ann N Y Acad Sci, 1098, 122-44.

Chrousos, G.P. & Gold, P.W. (1992). The concepts of stress and stress system disorders: Overview of physical and behavioral homeostasis. JAMA, 267(9), 1244-52. Erratum in JAMA (1992), 268(2), 200.

Kirschbaum, C., Read, G.F., & Hellhammer, D.H. (1994). Assessment of hormones and drugs in saliva in biobehavioral research. Göttingen: Hogrefe & Huber.

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Nater, U.M., & Rohleder, N. (2009). Salivary alpha-amylase as a non-invasive biomarker for the sympathetic nervous system: Current state of research. Psychoneuroendocrinology, 34(4), 486-96.

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van Stegeren, A., Rohleder, N., Everaerd, W., & Wolf, O.T. (2006). Salivary alpha amylase as marker for adrenergic activity during stress: Effect of betablockade. Psychoneuroendocrinology, 31(1), 137-41.

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Rohleder, N., Wolf, J.M., Maldonado, E.F., & Kirschbaum, C. (2006). The psychosocial stress-induced increase in salivary alpha-amylase is independent of saliva flow rate. Psychophysiology, 43(6), 645-52.

Bosch, J.A., Veerman, E.C., de Geus, E.J., & Proctor, G.B. (2011). α-Amylase as a reliable and convenient measure of sympathetic activity: Don’t start salivating just yet! Psychoneuroendocrinology, 36(4), 449-53.

Thoma, M.V., Kirschbaum, C., Wolf, J.M., & Rohleder, N. (2012). Acute stress responses in salivary alpha-amylase predict increases of plasma norepinephrine. Biol Psychol, 91(3), 342–48.

Granger, D. A., Kivlighan, K. T., Blair, C., El-Sheikh, M., Mize, J., Lisonbee, J.A., Buckhalt, J. A., et al. (2006). Integrating the measurement of salivary alpha-amylase into studies of child health, development, and social relationships. J Soc Pers Relat, 23(2), 267-90.

Segal, S.K., & Cahill, L. (2009). Endogenous noradrenergic activation and memory for emotional material in men and women. Psychoneuroendocrinology, 34(9), 1263-71.

Susman, E.J., Dockray, S., Granger, D.A., Blades, K.T., Randazzo, W., Heaton, J.A., & Dorn, L.D. (2010). Cortisol and alpha amylase reactivity and timing of puberty: Vulnerabilites for antisocial behaviour in young adolescents. Psychoneuroendocrinology, 35(4), 557-69.

Wallenfels, K., Foldi, P., Niermann, H., Bender, H., Linder, D. (1978). The enzymic synthesis, by transglucosylation of a homologous series of glycosidically substituted malto-oligosaccharides, and their use as amylase substrates. Carbohyd Res, 61(1), 359-68.

Weiner, D., Levy, Y., Khankin, E.V., Reznick, A.Z. (2008). Inhibition of salivary amylase activity by cigarette smoke aldehydes. J Physiol Pharmacol, 59(Suppl 6), 727-37.

Klein, L.C., Bennett, J.M., Whetzel, C.A., Granger, D.A., & Ritter, F.E. (2010). Caffeine and stress alter salivary α-amylase activity in young men. Human Psychopharmacol, 25(5), 359-67.

Nater, U.M., Rohleder, N., Scholtz, W., Ehlert, U., & Kirschbaum, C. (2007). Determinants of the diurnal course of salivary alpha-amylase. Psychoneuroendocrinology, 32(4), 392-401.

Mackie, D.A. & Pangborn, R.M. (1990). Mastication and its influence on human salivary flow and alpha-amylase secretion. Physiol Behav, 47(3), 593-95.

Lo Piparo, E., Scheib, H., Frei, N., Williamson, G., Grigorov, M., & Chou, C.J. (2008). Flavonoids for controlling starch digestion: Structural requirements for inhibiting human α-amylase. J Med Chem, 51(12), 3555-61.

Hara, K., Ohara, M., Hayashi, I., Hino, T., Nishimura, R., Iwasaki, Y., Ogawa, T., et al. (2012). The green tea polyphenol epigallocatechin gallate precipitates salivary proteins including alpha-amylase: Biochemical implications for oral health. Eur J Oral Sci, 120(2), 132-39.

Beltzer, E.K., Fortunato, C.K., Guaderrama, M.M., Peckins, M.K., Garramone, B.M., & Granger, D.A. ( 2010). Salivary flow and alpha-amylase: Collection technique, duration, and oral fluid type. Physiol Behav, 101(2), 289-96.

Harmon, A.G., Towe-Goodman, N.R., Fortunato, C.K., & Granger, D.A. (2008). Differences in saliva collection location and disparities in baseline and diurnal rhythms of alpha-amylase: A preliminary note of caution. Horm Behav, 54(5), 592-96.

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